Now in a revised and updated edition, this book provides biological sciences professionals with the fundamentals of contemporary mass spectrometry so that they can confidently choose and apply the best instrumentation and experimental approaches for their needs. The new edition now includes metabolomics, proteomics, protein-protein interactions and networks, MALDI, ESI, and nanoESI, as well as more applications for real-world problems. The only single-volume guide available, this text combines coverage of the latest instrumentation, techniques, and applications, making it an excellent working resource for experts and novices alike.