This book LNCS 16213 constitutes the refereed proceedings of the 29th International Conference on Enterprise Design, Operations, and Computing, EDOC 2025, held in Lisbon, Portugal, during September 9–12, 2025.

The 19 full papers were carefully reviewed and seleced from 73 submissions. They were categorized under topical sections as follows:

1) Enterprise Architecture

2) Business Process Management

3) Business Processes & Decision-Making

4) Modeling and Modeling Methods

5) Ontologies

6) Artificial Intelligence and Agents