This sixth edition explores the tools used to acquire, process, and analyze data contained in images, for a wide variety of scientific fields. Using new examples and diagrams, it presents new sections on selecting a camera with resolution appropriate for use on light microscopes, the ability of current cameras to capture raw images with high dynamic range, and imaging in more than two dimensions. The book also discusses Dmax for x-ray images and combining images with different exposure settings to further extend the dynamic range. New coverage also includes shape measurements and developments in image file searching.