The Ayurveda products have been found effective in many rare diseases, but the standardization gives demerit to these products. The effective standardization protocol remains a bottleneck for drug discovery scientists. In the sequence of the Ayurveda formulation, the polyherbal Churna has been tried for the development of the churna method. The Central Council for Researching in the Ayurveda field has guided for the general standardization, which is taken as a reference for the standardization protocol. Herbal formulation standardization are necessary for accessing the quality of drugs belongs to the concentration of active principles, physical and chemical standards. In the present work standardization of churna of poly herbal formulations used for anxiolytic is reported. Also, the marketed sample that was used commercially was matched with the prepared formulation and the same results were obtained after standardization. The parameter was selected and noted enough for evaluating the study of churna and reference standard for the quality control and assuring purpose.