Emergence of multi-resistant organisms (MROs) leads to ineffective treatment with the currently available medications which pose a great threat to public health and food technology sectors. In this regard, there is an urgent need to strengthen the present therapies or to look over for other prospective alternatives such as use of "metal nanocomposites" and "drug loaded polymeric nanofibers". Herein, we report synthesis of silver-zinc oxide (Ag-ZnO) nanocomposites and drug loaded chitosan-PEO (Polyethylene oxide) nanofibers with excellent antibacterial activity. Formulation of such nanocomposites and nanofibers hold great promise towards development of antimicrobial packages and for various biomedical applications.