The practical aspects of analyzing rumen liquor for the proportion of VFAs enzymatic activity of various metabolites, as well as estimating rumen fluid volume and its flow rate, are explored in great detail. Furthermore, the manual covers the estimation of anti-nutritional or toxic factors in various unconventional feeds using HPLC/Spectrophotometer, as well as detailed analysis of milk and body condition scoring for dairy cattle. These parameters are crucial in Ruminant Nutrition Research, and the manual is designed for postgraduate students, teachers, researchers, and feed industry professionals. The manual does not include exhaustive details, as the primary focus is on providing practical information for those in the field.