The manual provides extensive coverage of various aspects of ruminant nutrition research, including the analysis of rumen liquor for the fraction of VFAs enzymatic activity of various metabolites, the estimation of rumen fluid volume and its flow rate, and the estimation of anti-nutritional/toxic factors in various unconventional feeds using HPLC/Spectrophotometer. Additionally, the manual includes detailed analysis of milk and body condition scoring for dairy cattle, as these parameters are crucial in ruminant nutrition research. It should be noted that the manual has been designed primarily for postgraduate scholars, teachers, scientists, and feed industry personnel, and as such, the exhaustive details have been intentionally avoided.