The present study was undertaken to determine standard plate count (SPC) for total bacteria, lactic acid bacterial count (LABC), lipolytic bacterial count (LBC), Proteolytic bacterial count (PBC) and coliforms for determining bacterial quality of raw milk as influenced by metabolic size of healthy crossbred cows by dry full hand method of milking at SHUATS dairy farm Prayagraj. The analysis of variance showed significant effect of metabolic size on SPC, LBC, and coliforms but showed no significant differences in LABC and PBC in raw milk. The result indicated that the overall rating of bacterial quality was found better for raw milk produced by 262 to 296, and 297 to 333 followed by cows of 190 to 225 and 226 to 261 metabolic size. If the quality is judged on the basis of coliform than the quality of milk of was better from cows of metabolic size 226 to 261 and 297 to 333 followed by cows of 262 to 296 and 190 to 225 metabolic size.