The two-volume proceedings set LNCS 13177 and 13178 constitutes the refereed proceedings of the 25th IACR International Conference on Practice and Theory of Public Key Cryptography, PKC 2022, which took place virtually during March 7-11, 2022. The conference was originally planned to take place in Yokohama, Japan, but had to change to an online format due to the COVID-19 pandemic.The 40 papers included in these proceedings were carefully reviewed and selected from 137 submissions. They focus on all aspects of public-key cryptography, covering cryptanalysis; MPC and secret sharing; cryptographic protocols; tools; SNARKs and NIZKs; key exchange; theory; encryption; and signatures.