This book contains the manuscripts that were accepted for publication in the MDPI Special Topic "Cyber Security and Critical Infrastructure" after a rigorous peer-review process. Authors from academia, government and industry contributed their innovative solutions, consistent with the interdisciplinary nature of cybersecurity. The book contains 16 articles: an editorial explaining current challenges, innovative solutions, real-world experiences including critical infrastructure, 15 original papers that present state-of-the-art innovative solutions to attacks on critical systems, and a review of cloud, edge computing, and fog's security and privacy issues.