Giardiasis is a zoonotic parasitic disease caused by the flagellate protozoan Giardia. It is the most common pathogenic parasitic infection in humans worldwide. The infection occurs in many animals and animals are believed to play a role in keeping infections present in an environment Giardiasis is transmitted via the fecal-oral route with the ingestion of cysts.Primary routes are personal contact and contaminated water and food. Owing to its zoonotic implication the present study was planned to study the epidemiological pattern of giardiosis in diarrhoeic bovine calves and dogs and prevalence of giardiosis in humans associated with dogs and calves, also a comparison was made between coproantigen ELISA with conventional diagnostic tests.